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font-size:22px; margin:0px;"><u>Biological diagnosis of Human Immunodeficiency Virus (HIV)</u></h1></B></FONT></td> </tr> <tr> <td style="WORD-BREAK: break-all" colSpan=3><h2 style=" font-family:Arial; font-size:16px; margin:0px;"><i>Diagnostic biologique des virus de immunodéficience humaine (VIH)</i></h2></td> </tr> <tr> <td colSpan=3>&nbsp;</td> </tr> <tr> <td height=23 colSpan=3 class=Article_tdbgall> <table width="100%"> <tr> <td width="58%"><FONT size=2><i>Author: <a href='/ShowAuthor.asp?ChannelID=1&AuthorName=BeautyAngle' title='BeautyAngle'>BeautyAngle</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Reads: <script language='javascript' src='http://beautyangleshop.com/Article/GetHits.asp?ArticleID=795'></script></i></FONT> </td> <td width="42%" align="right"><FONT size=1><!-- AddThis Button BEGIN --> <a href="http://www.addthis.com/bookmark.php?v=250" onMouseOver="return addthis_open(this, '', '[URL]', '[TITLE]')" onMouseOut="addthis_close()" onClick="return addthis_sendto()"><img src="http://s7.addthis.com/static/btn/lg-share-en.gif" width="125" height="16" alt="Bookmark and Share" style="border:0"/></a><script type="text/javascript" src="http://s7.addthis.com/js/250/addthis_widget.js?pub=beautyangle"></script> <!-- AddThis Button END --> <a href='http://beautyangleshop.com/Article/Comment.asp?ArticleID=795' target='_blank'>Comment</a> <a href='/User/User_Favorite.asp?Action=Add&ChannelID=1&InfoID=795' target='_blank'>Favorite</a> <a href='http://beautyangleshop.com/Article/SendMail.asp?ArticleID=795' target='_blank'>SendMail</a> <a href='http://beautyangleshop.com/Article/Print.asp?ArticleID=795' target='_blank'>Print</a> <a href='javascript:window.close();'>Close</a> </FONT></td> </tr> </table> </td> </tr> <tr> <td class=bgc1 colSpan=3 height=1></td> </tr> <tr> <td valign="top" id=fontzoom height=300><P><STRONG><U>Biological diagnosis of Human Immunodeficiency Virus (HIV)</U></STRONG></P> <P><STRONG></STRONG><STRONG><A title="Biological diagnosis of Human Immunodeficiency Virus (HIV)" href="http://www.beautyangleshop.com/Article" target=_blank><IMG onmousewheel="return bbimg(this)" alt="" hspace=0 src="http://beautyangleshop.com/Article/UploadFiles/200909/2009090908402683.jpg" onload=resizepic(this) align=left border=0></A>A. Morphology <BR></STRONG>Human immunodeficiency virus is about 120 nanometers in diameter, roughly spherical. Viral membrane is the phospholipid bilayer from the host cell, and embedded a virus protein gp120 and gp41; gp41 is a transmembrane protein, gp120 at the surface, and with the role of gp41 through non-covalent binding. Inward by the p17 protein in the formation of spherical matrix (Matrix), and protein p24 to form the semi-conical capsid (Capsid), capsid at the electron microscope showed a high electron density. Capsid containing the virus RNA genome, enzymes (reverse transcriptase, integrase, protease), and other components from the host cell (such as tRNAlys3, as the reverse primer). </P> <P><STRONG>B. Gene structure and function of proteins encoded <BR></STRONG>The virus genome is just the same two RNA, each RNA is about 9.2-9.8kb. At both ends of long terminal repeat (long terminal repeats, LTR), contains cis-regulatory sequences to control the proviral expression. Has proved to have started in the LTR promoter and enhancer and containing the negative regulatory region. LTR sequence encoding at least among the nine proteins can be divided into Triple categories: structural proteins, regulatory proteins, auxiliary protein. <BR><BR>1. gag genes encoding about 500 amino acids of the polymer precursor protein by proteolysis to form P17, P24 nucleoprotein to RNA from nuclease damage the outside world. <BR><BR>2. Pol gene encoding polymerase precursor protein, after cutting the formation of protease, integrase, reverse transcriptase, RNase H, are necessary for viral proliferation. <BR><BR>3. env gene encodes about 863 amino acid precursor protein, and sugar-based chemical gp160, gp120 and gp41. gp120 contains a neutralization epitope, has proved to be HIV neutralization epitope on the gp120 V3 loop, V3 loop area is a major envelope protein functional area, in the virus and cell fusion plays an important role. gp120 and the transmembrane protein gp41 of non-covalent bond. gp41 fusion with target cells, prompting the virus into the cell. Experiments show that gp41 is also a strong antigen, can induce antibody response. <BR><BR>4. TaT gene encoding protein can be combined with the LTR to increase viral gene transcription rates for all, but also in the post-transcriptional promote viral mRNA translation. </P> <P><FONT color=#ff0000></p><p align='center'><b><font color='red'>[1]</font> <a href='http://beautyangleshop.com/Article/Health/AIDS/200909/795_2.html'>[2]</a> <a href='http://beautyangleshop.com/Article/Health/AIDS/200909/795_3.html'>[3]</a> <a href='http://beautyangleshop.com/Article/Health/AIDS/200909/795_2.html'> Next </a> </b></p> <CENTER></CENTER></td> <td valign="top"></td> <td align="center" vAlign=top> <script language='javascript' src='/AD/200905/23.js'></script></td> </tr> <tr> <td colspan="4" align="right" valign="bottom"><div id="fb-root"></div><script src="http://connect.facebook.net/en_US/all.js#xfbml=1"></script><fb:like href="http://www.beautyangleshop.com/Article/ShowArticle.asp?ArticleID=795" send="true" width="450" show_faces="true" action="like" font="tahoma"></fb:like> </td> </tr> <tr> <td align="center" colSpan=4 height=10> <script language='javascript' src='/AD/200810/2.js'></script></td> </tr> <tr> <td colSpan=3 height=6></td> </tr> <tr> <td style="font-family:Arial;" colSpan=3><em><li>Prev : <a class='LinkPrevArticle' href='http://beautyangleshop.com/Article/Health/AIDS/200909/794.html' title=' Title: Human Immunodeficiency Virus (HIV) by:BeautyAngle Updated:9/9/2009 8:25:12 AM'>Human Immunodeficiency Virus (HIV)</a></li></em></td> </tr> <tr> <td style="font-family:Arial;" colSpan=3><em><li>Next : <a class='LinkNextArticle' href='http://beautyangleshop.com/Article/Health/AIDS/201001/1169.html' title=' Title: Self-determine whether the AIDS patients by:Anon. 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demo1=document.getElementById("demo1"); demo2.innerHTML=demo1.innerHTML function Marquee(){ if(demo2.offsetTop-demo.scrollTop<=0) demo.scrollTop-=demo1.offsetHeight else{ demo.scrollTop++ } } var MyMar=setInterval(Marquee,speed) demo.onmouseover=function() {clearInterval(MyMar)} demo.onmouseout=function() {MyMar=setInterval(Marquee,speed)} </script><!--T NÚn¨RãNxÓ~_g--></td> <td></td> </tr> <tr> <td bgcolor="#aaaaaa" height=28></td> <td bgcolor="#aaaaaa"><strong>Tags:</strong></td> <td bgcolor="#aaaaaa"></td> </tr> <tr> <td height=28></td> <td style="font-family:Arial;"><em><strong>Human Immunodeficiency Virus, HIV, Biological diagnosis</strong></em></td> <td></td> </tr> <tr> <td height=8></td><td></td><td></td> </tr> <tr> <td bgcolor="#aaaaaa" width=13 height=28></td> <td bgcolor="#aaaaaa"><FONT size=2><B>Related Articles</B></FONT></td> <td bgcolor="#aaaaaa" width=10></td> </tr> </table> <table cellSpacing=0 cellPadding=0 width="100%" border=0> <tr> <td width=1></td> <td vAlign=top> <table style="WORD-BREAK: break-all" cellSpacing=0 cellPadding=0 width="96%" align=center border=0> <tr> <td style="font-family:Arial;" vAlign=top height=70><FONT size=2><em><a class='LinkArticleCorrelative' href='http://beautyangleshop.com/Article/Health/AIDS/200909/794.html' title=' Title: Human Immunodeficiency Virus (HIV) by:BeautyAngle Updated:9/9/2009 8:25:12 AM' target="_self">Human Immunodeficiency Virus (HIV)</a><br></em></FONT></td> </tr> </table> </td> <td width=1</td> </tr> </table> </td> <td width=5></td> <td class=bgc width=8></td> </tr> </table> <table class=bgc cellSpacing=0 cellPadding=0 width=1000 align=center border=0> <tr> <td class=bgc width=8></td> <td vAlign=top> <table cellSpacing=0 cellPadding=0 width="100%" border=0> <tr> <td bgColor=#ffffff height=5></td> </tr> <tr> <td bgColor=#f8cdfa height=1></td> </tr> <tr> <td bgColor=#ffffff height=5></td> </tr> <tr> <td bgColor=#f8cdfa height=1></td> </tr> <tr> <td bgColor=#ffffff height=5></td> </tr> </table> <table 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